Knockdown of TRIM65 inhibits autophagy and cisplatin resistance in A549/DDP cells by regulating miR-138-5p/ATG7
Identifieur interne : 000702 ( Main/Exploration ); précédent : 000701; suivant : 000703Knockdown of TRIM65 inhibits autophagy and cisplatin resistance in A549/DDP cells by regulating miR-138-5p/ATG7
Auteurs : Xufeng Pan ; Yong Chen ; Yuzhou Shen ; Jicheng TantaiSource :
- Cell Death & Disease [ 2041-4889 ] ; 2019.
Abstract
Cisplatin resistance is the main cause of treatment failure in patients with non-small-cell lung cancer (NSCLC). Autophagy is a key mechanism of resistance to chemotherapy. Given that tripartite motif (TRIM)-containing proteins are involved in the regulation of autophagy and chemoresistance, we aimed to study the functions of TRIM protein members in autophagy-mediated chemoresistance of NSCLC. We found that TRIM65 was significantly increased in cisplatin-resistant NSCLC cell line (A549/DDP) as compared to the parental cell line (A549). Knockdown of TRIM65 can enhance cisplatin-induced apoptosis and inhibit autophagy in A549/DDP cells, as indicated by Annexin V/PI staining, caspase3 activity test, and LC3-II immunofluorescence staining. Additionally, knockdown of TRIM65 significantly decreased the expression of an important autophagy mediator, ATG7, which was a potential target of miR-138-5p. miR-138-5p inhibitor significantly abolished the effects of TRIM65 knockdown on autophagy and cisplatin-induced apoptosis. Moreover, TRIM65 induced the ubiquitination and degradation of TNRC6A, resulting in the suppressed expression of miR-138-5p. TRIM65 knockdown inhibited the growth of tumors derived from A549/DDP cells. Furthermore, cisplatin-resistant NSCLC tissues displayed higher expression of TRIM65 mRNA and lower expression of miR-138-5p as compared to cisplatin non-resistant ones. miR-138-5p expression was negatively correlated with TRIM65 mRNA in NSCLC tissues. Collectively, the present study indicates that TRIM65 knockdown attenuates autophagy and cisplatin resistance in A549/DDP cells via regulating miR-138-5p.
Url:
DOI: 10.1038/s41419-019-1660-8
PubMed: 31160576
PubMed Central: 6546683
Affiliations:
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Autophagy is a key mechanism of resistance to chemotherapy. Given that tripartite motif (TRIM)-containing proteins are involved in the regulation of autophagy and chemoresistance, we aimed to study the functions of TRIM protein members in autophagy-mediated chemoresistance of NSCLC. We found that TRIM65 was significantly increased in cisplatin-resistant NSCLC cell line (A549/DDP) as compared to the parental cell line (A549). Knockdown of TRIM65 can enhance cisplatin-induced apoptosis and inhibit autophagy in A549/DDP cells, as indicated by Annexin V/PI staining, caspase3 activity test, and LC3-II immunofluorescence staining. Additionally, knockdown of TRIM65 significantly decreased the expression of an important autophagy mediator, ATG7, which was a potential target of miR-138-5p. miR-138-5p inhibitor significantly abolished the effects of TRIM65 knockdown on autophagy and cisplatin-induced apoptosis. Moreover, TRIM65 induced the ubiquitination and degradation of TNRC6A, resulting in the suppressed expression of miR-138-5p. TRIM65 knockdown inhibited the growth of tumors derived from A549/DDP cells. Furthermore, cisplatin-resistant NSCLC tissues displayed higher expression of TRIM65 mRNA and lower expression of miR-138-5p as compared to cisplatin non-resistant ones. miR-138-5p expression was negatively correlated with TRIM65 mRNA in NSCLC tissues. 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sortKey="L Pez, P" uniqKey="L Pez P">P López</name></author><author><name sortKey="Chulvi, R" uniqKey="Chulvi R">R Chulvi</name></author><author><name sortKey="Ca Abate, M" uniqKey="Ca Abate M">M Cañabate</name></author><author><name sortKey="Dolores, T" uniqKey="Dolores T">T Dolores</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chang, A" uniqKey="Chang A">A Chang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mizushima, N" uniqKey="Mizushima N">N Mizushima</name></author><author><name sortKey="Komatsu, M" uniqKey="Komatsu M">M Komatsu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kobayashi, S" uniqKey="Kobayashi S">S Kobayashi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wu, Wk" uniqKey="Wu W">WK Wu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sui, X" uniqKey="Sui X">X Sui</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Aredia, F" uniqKey="Aredia F">F Aredia</name></author><author><name sortKey="Scovassi, Ai" uniqKey="Scovassi A">AI Scovassi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Goldberg, Sb" uniqKey="Goldberg S">SB Goldberg</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lin, S" uniqKey="Lin S">S Lin</name></author><author><name sortKey="Gregory, Ri" uniqKey="Gregory R">RI Gregory</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pink, Rc" uniqKey="Pink R">RC Pink</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xia, L" uniqKey="Xia L">L Xia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liang, Z" uniqKey="Liang Z">Z Liang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Q" uniqKey="Wang Q">Q Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Seca, H" uniqKey="Seca H">H Seca</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jin, F" uniqKey="Jin F">F Jin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xu, L" uniqKey="Xu L">L Xu</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Ozato, K" uniqKey="Ozato K">K Ozato</name></author><author><name sortKey="Shin, D M" uniqKey="Shin D">D-M Shin</name></author><author><name sortKey="Chang, T H" uniqKey="Chang T">T-H Chang</name></author><author><name sortKey="Morse, Hc" uniqKey="Morse H">HC Morse</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hatakeyama, S" uniqKey="Hatakeyama S">S Hatakeyama</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hatakeyama, S" uniqKey="Hatakeyama S">S Hatakeyama</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, L" uniqKey="Zhang L">L Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ni, M" uniqKey="Ni M">M Ni</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y Wang</name></author><author><name sortKey="Xie, L" uniqKey="Xie L">L Xie</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, Y" uniqKey="Liu Y">Y Liu</name></author><author><name sortKey="Zhang, B" uniqKey="Zhang B">B Zhang</name></author><author><name sortKey="Shi, T" uniqKey="Shi T">T Shi</name></author><author><name sortKey="Qin, H" uniqKey="Qin H">H Qin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhao, T T" uniqKey="Zhao T">T-T Zhao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tan, Z" uniqKey="Tan Z">Z Tan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yu, C" uniqKey="Yu C">C Yu</name></author><author><name sortKey="Chen, S" uniqKey="Chen S">S Chen</name></author><author><name sortKey="Guo, Y" uniqKey="Guo Y">Y Guo</name></author><author><name sortKey="Sun, C" uniqKey="Sun C">C Sun</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, S" uniqKey="Li S">S Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tanida, I" uniqKey="Tanida I">I Tanida</name></author><author><name sortKey="Ueno, T" uniqKey="Ueno T">T Ueno</name></author><author><name sortKey="Kominami, E" uniqKey="Kominami E">E Kominami</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Qin, X" uniqKey="Qin X">X Qin</name></author><author><name sortKey="Yu, S" uniqKey="Yu S">S Yu</name></author><author><name sortKey="Xu, X" uniqKey="Xu X">X Xu</name></author><author><name sortKey="Shen, B" uniqKey="Shen B">B Shen</name></author><author><name sortKey="Feng, J" uniqKey="Feng J">J Feng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Xl" uniqKey="Wang X">XL Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, Zj" uniqKey="Yang Z">ZJ Yang</name></author><author><name sortKey="Chee, Ce" uniqKey="Chee C">CE Chee</name></author><author><name sortKey="Huang, S" uniqKey="Huang S">S Huang</name></author><author><name sortKey="Sinicrope, Fa" uniqKey="Sinicrope F">FA Sinicrope</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, N" uniqKey="Zhang N">N Zhang</name></author><author><name sortKey="Yang, G" uniqKey="Yang G">G Yang</name></author><author><name sortKey="Shao, X" uniqKey="Shao X">X Shao</name></author><author><name sortKey="Wei, L" uniqKey="Wei L">L Wei</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jin, Z" uniqKey="Jin Z">Z Jin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhao, X" uniqKey="Zhao X">X Zhao</name></author><author><name sortKey="Yang, L" uniqKey="Yang L">L Yang</name></author><author><name sortKey="Hu, J" uniqKey="Hu J">J Hu</name></author><author><name sortKey="Ruan, J" uniqKey="Ruan J">J Ruan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tian, S" uniqKey="Tian S">S Tian</name></author><author><name sortKey="Guo, X" uniqKey="Guo X">X Guo</name></author><author><name sortKey="Yu, C" uniqKey="Yu C">C Yu</name></author><author><name sortKey="Sun, C" uniqKey="Sun C">C Sun</name></author><author><name sortKey="Jiang, J" uniqKey="Jiang J">J Jiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Deng, X S" uniqKey="Deng X">X-S Deng</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Chen, Yong" sort="Chen, Yong" uniqKey="Chen Y" first="Yong" last="Chen">Yong Chen</name><name sortKey="Pan, Xufeng" sort="Pan, Xufeng" uniqKey="Pan X" first="Xufeng" last="Pan">Xufeng Pan</name><name sortKey="Shen, Yuzhou" sort="Shen, Yuzhou" uniqKey="Shen Y" first="Yuzhou" last="Shen">Yuzhou Shen</name><name sortKey="Tantai, Jicheng" sort="Tantai, Jicheng" uniqKey="Tantai J" first="Jicheng" last="Tantai">Jicheng Tantai</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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